Myc Epitope Tag Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43691
Key Product Details
Species Reactivity
Epitope Tag
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide corresponding to a region within amino acids 410 and 419 of human c-Myc
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Myc Epitope Tag Antibody
Western Blot: Myc Epitope Tag Antibody [NBP2-43691]
Western Blot: Myc Epitope Tag Antibody [NBP2-43691] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Myc tag antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody [NBP2-43691]
Immunocytochemistry/Immunofluorescence: Myc Epitope Tag Antibody [NBP2-43691] - Analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled (red) at 1:2500.Western Blot: Myc Epitope Tag Antibody [NBP2-43691]
Western Blot: Myc Epitope Tag Antibody [NBP2-43691] - Analysis of extracts from untransfected control cells and transfected cells overexpressing Myc tagged protein, using Myc tag antibody. A: Non-transfected 293T lysate (10 ug) B: Transfected 293T lysate (10 ug) (~60 kDa) .Applications for Myc Epitope Tag Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:10000
Western Blot
1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Myc Epitope Tag
Alternate Names
BHLHE39, Class E basic helix-loop-helix protein 39 (bHLHe39), Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64
Additional Myc Epitope Tag Products
Product Documents for Myc Epitope Tag Antibody
Product Specific Notices for Myc Epitope Tag Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.