MYCL1/L-Myc Antibody (PCRP-MYCL-2D5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13977

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MYCL-2D5

Concentration

0.2 mg/ml

View all MYCL1/L-Myc Antibodies »

Product Specifications

Immunogen

Recombinant full-length human MYCL1/L-Myc protein (Uniprot: P12524)

Localization

Nucleus, Nucleoplasm, Mitotic chromosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

40.33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14011)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MYCL1/L-Myc Antibody (PCRP-MYCL-2D5)

Immunocytochemistry/ Immunofluorescence: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977]

Immunocytochemistry/ Immunofluorescence: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977]

Immunocytochemistry/Immunofluorescence: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977]

Flow Cytometry: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977]

Flow Cytometry: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977] - Flow cytometric analysis of PFA-fixed HeLa cells. MYCL1/L-Myc antibody (PCRP-MYCL-2D5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977]

Protein Array: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977]

Protein Array: MYCL1/L-Myc Antibody (PCRP-MYCL-2D5) [NBP3-13977] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MYCL1/L-Myc Antibody (PCRP-MYCL-2D5).

Applications for MYCL1/L-Myc Antibody (PCRP-MYCL-2D5)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MYCL1/L-Myc

MYCL1 is a member of the MYC family that was isolated from small cell lung carcinoma. Like the other members of the MYC family, MYCL1 is a proto-oncogene transcription factor belonging to the helix-loop-helix basic leucine zipper (HLH bzip) family. With its heterodimeric partners, MYCL1 binds to the DNA consensus site 5'-CACGTG-3'. MYCL1 is implicated in controlling a wide range of cellular processes from cellular proliferation to apoptosis.

Long Name

v-Myc Myelocytomatosis Viral Oncogene Homolog 1, Lung Carcinoma Derived

Alternate Names

L-Myc, LMyc

Gene Symbol

MYCL

UniProt

P12524

Additional MYCL1/L-Myc Products

Product Documents for MYCL1/L-Myc Antibody (PCRP-MYCL-2D5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MYCL1/L-Myc Antibody (PCRP-MYCL-2D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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