Immunocytochemistry/Immunofluorescence: MyD88 Antibody (4D6) [NBP2-27369] - Analysis of MyD88 antibody in MCF-7 cells using an isotype control (top) and MyD88 Antibody (bottom) at 5 ug/ml.

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on Jurkat cells with MyD88 Antibody (4D6) NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Western Blot: MyD88 Antibody (4D6)BSA Free [NBP2-27369]

Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Analysis of MyD88 in A) human ovary, B) human prostate and Jurkat cell lysate in the C) absence and D) presence of immunizing peptide using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - Intracellular analysis of MyD88 antibody in Jurkat cells using 0.5 ug. Shaded histogram represents cells without antibody; green represents isotype control; red represents MyD88 antibody. This antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.

Flow (Intracellular): MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow (Intracellular): MyD88 Antibody (4D6) [NBP2-27369] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-27369. Staining of MyD88 antibody in Jurkat cells using 10 ul (0.5 ug) of MyD88 antibody. Green represents isotype control (20108); red represents anti-MyD88 antibody.

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 (4D6) antibody NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 [4D6] Antibody NBP2-27369AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on Jurkat cells with MyD88 Antibody (4D6) NBP2-27369 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on U-937 cells with MyD88 Antibody (4D6) NBP2-27369 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 Antibody (4D6) NBP2-27369F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

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Applications for MyD88 Antibody (4D6) - BSA Free

Application

Recommended Usage

Flow Cytometry

0.5ug/10^6cells~

Immunocytochemistry/ Immunofluorescence

5ug/ml

Western Blot

2 ug/ml

Application Notes

This antibody is Cytof ready.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-27369 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MyD88

MyD88, a protein involved in interleukin-1 (IL-1) mediated signalling was originally isolated as myeloid differentiation primary response gene (3,4). MyD88 possesses a N-terminal death domain similar to cytoplasmic segments of TNF receptor 1, Fas, and C-terminal region related to IL-1 and Toll receptors. Overexpression of MyD88 induces activation the c-Jun N-terminal kinase and NF-kB through its death domain (1,2). The C-terminus of MyD88 interacts with the IL-1 receptor and blocks NF-kB activation induced by IL-1, but not by NF-kB.

Long Name

Myeloid Differentiation Primary Response Gene 88

Alternate Names

MYD88D, myeloid differentiation primary response gene (88), myeloid differentiation primary response protein MyD88

Gene Symbol

MYD88

Additional MyD88 Products

Product Documents for MyD88 Antibody (4D6) - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for MyD88 Antibody (4D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

MyD88 Antibody (4D6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27369

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all MyD88 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Specificity

Clonality

Host

Isotype

Scientific Data Images for MyD88 Antibody (4D6) - BSA Free

Western Blot: MyD88 Antibody (4D6)BSA Free [NBP2-27369]

Immunocytochemistry/ Immunofluorescence: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Western Blot: MyD88 Antibody (4D6)BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow (Intracellular): MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369]

Applications for MyD88 Antibody (4D6) - BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: MyD88

Long Name

Alternate Names

Gene Symbol

Additional MyD88 Products

Product Documents for MyD88 Antibody (4D6) - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for MyD88 Antibody (4D6) - BSA Free