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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 25 and 50 of human Myelin Expression Factor 2 using the numbering given in entry NP_057216.2 (GeneID 50804).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myelin expression factor 2 Antibody

Immunoprecipitation: Myelin expression factor 2 Antibody [NBP2-12765]

Immunoprecipitation: Myelin expression factor 2 Antibody [NBP2-12765]

Immunoprecipitation: Myelin expression factor 2 Antibody [NBP2-12765] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MYEF2 antibody NBP2-12764 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MYEF2 was also immunoprecipitated by rabbit anti-MYEF2 antibody NBP2-12765, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for Myelin expression factor 2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Myelin expression factor 2

Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA

Alternate Names

FLJ11213, HsT18564, KIAA1341MSTP156, MEF-2myEF-2, MGC87325, MST156, MyEF-2, myelin expression factor 2, myelin gene expression factor 2

Gene Symbol

MYEF2

Additional Myelin expression factor 2 Products

Product Documents for Myelin expression factor 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myelin expression factor 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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