Myelin expression factor 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12682
![Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]](https://resources.bio-techne.com/images/products/Myelin-expression-factor-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12682-img0002.jpg "Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]")
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human Myelin expression factor 2 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for Myelin expression factor 2 Antibody
![Western Blot: Myelin expression factor 2 Antibody [NBP3-12682]](https://resources.bio-techne.com/images/products/Myelin-expression-factor-2-Antibody-Western-Blot-NBP3-12682-img0001.jpg "Western Blot: Myelin expression factor 2 Antibody [NBP3-12682]")
Western Blot: Myelin expression factor 2 Antibody [NBP3-12682]
Western Blot: Myelin expression factor 2 Antibody [NBP3-12682] - Lane A: SH-SY5Y Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:64 kDaObserved band size:70 kDa![Immunocytochemistry/ Immunofluorescence: Myelin expression factor 2 Antibody [NBP3-12682]](https://resources.bio-techne.com/images/products/Myelin-expression-factor-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12682-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Myelin expression factor 2 Antibody [NBP3-12682]")
Immunocytochemistry/ Immunofluorescence: Myelin expression factor 2 Antibody [NBP3-12682]
Immunocytochemistry/Immunofluorescence: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of MYEF2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MYEF2 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.![Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]](https://resources.bio-techne.com/images/products/Myelin-expression-factor-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12682-img0004.jpg "Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]")
Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]
Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682] - Staining of human MYEF2 in human kidney with rabbit polyclonal antibody at 1:10000 dilution.![Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]](https://resources.bio-techne.com/images/products/Myelin-expression-factor-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12682-img0003.jpg "Immunohistochemistry-Paraffin: Myelin expression factor 2 Antibody [NBP3-12682]")
![Immunoprecipitation: Myelin expression factor 2 Antibody [NBP3-12682]](https://resources.bio-techne.com/images/products/Myelin-expression-factor-2-Antibody-Immunoprecipitation-NBP3-12682-img0006.jpg "Immunoprecipitation: Myelin expression factor 2 Antibody [NBP3-12682]")
Applications for Myelin expression factor 2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:1000-1:20000
Immunoprecipitation
1-5uL/mg of uLysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Myelin expression factor 2
Alternate Names
FLJ11213, HsT18564, KIAA1341MSTP156, MEF-2myEF-2, MGC87325, MST156, MyEF-2, myelin expression factor 2, myelin gene expression factor 2
Gene Symbol
MYEF2
Additional Myelin expression factor 2 Products
Product Documents for Myelin expression factor 2 Antibody
Product Specific Notices for Myelin expression factor 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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