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Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08534CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Reptile

Applications

Flow Cytometry, Immunohistochemistry-Paraffin

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2A Clone # PLP1/4259

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1]

Immunogen

Synthetic peptide GRGTKF that recognizes the C terminal region (aa272-277) of Myelin PLP protein. (Uniprot: P60201)

Reactivity Notes

Tenerife Lizard

Localization

Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Scientific Data Images for Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1]

Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1]

Flow Cytometry: Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1] [NBP3-08534CL1]

Flow Cytometry: Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1] [NBP3-08534CL1] - Total extracellular vesicles with Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] (Catalog # NBP3-08534AF647) (left). Gate determined vesicles positive for PLP1 antibody ranging from 500-800nm size. Image on the right represents the same sample without antibody. 10ul of pre-isolated extracellular vesicles were incubated with 2ul of Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] , during 20 minutes at room temperature, and analyzed in nanoscale flow cytometer able to resolve particles scattering as little as 100um. Image from a verified customer review.

Applications for Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1]

Application
Recommended Usage

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Myelin PLP

Human PLP (also known as lipophilin) is a 30 kDa protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in compaction. Different mutations of the human PLP gene product result in two neurological disorders - Pelizaeus-Merzbacher disease and Spastic Paraplegia type 2.

Long Name

Proteolipid Protein

Alternate Names

DM-20, Lipophilin, MMPL, PLP1, SPG2

Gene Symbol

PLP1

Additional Myelin PLP Products

Product Documents for Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myelin PLP Antibody (PLP1/4259) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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