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Myelin Protein Zero Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1607

Novus Biologicals, part of Bio-Techne
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NB100-1607

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Chickens were immunized with two synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of Myelin Protein Zero, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences.

Reactivity Notes

Human, Mouse reactivity reported in scientific literature (PMID: 26406915), Rat reactivity reported in scientific literature (PMID: 22511272).

Marker

Schwann Cell Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Myelin Protein Zero Antibody

Immunocytochemistry/ Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607]

Immunocytochemistry/ Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607]

Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.
Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607]

Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607]

Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.

Applications for Myelin Protein Zero Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000-1:5000

Immunohistochemistry

1:2000-1:5000

Western Blot

1:10000-1:20000
Application Notes
Western Blot reactivity reported in scientific literature (PMID: 22689911). Use in immunoprecipitation reported in scientific literature (PMID:26406915).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (0.9% isotonic, w/v, pH 7.2)

Preservative

0.02% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Myelin Protein Zero

Myelin Protein Zero encodes a major structural protein of peripheral myelin. Mutations in this gene result in the autosomal dominant form of Charcot-Marie-Tooth disease type 1 as other polyneuropathies. [provided by RefSeq]

Alternate Names

Charcot-Marie-Tooth neuropathy 1B, CHM, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, HMSNIB, MPP, Myelin peripheral protein, myelin protein P0, myelin protein zeroDSS, P0

Entrez Gene IDs

4359 (Human); 17528 (Mouse)

Gene Symbol

MPZ

Additional Myelin Protein Zero Products

Product Documents for Myelin Protein Zero Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myelin Protein Zero Antibody

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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