Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72846
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0004.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
![Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3)Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Western-Blot-NBP2-72846-img0001.jpg "Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3)Azide and BSA Free [NBP2-72846]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3A3
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human MLF1(NP_071888) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
30.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free
Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3)Azide and BSA Free [NBP2-72846]
Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Myeloid leukemia factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with a![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0009.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]
Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Human pancreas tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.![Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72846-img0003.jpg "Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]
Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Myeloid leukemia factor 1.![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0005.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0006.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0007.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0008.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0010.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
![Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]](https://resources.bio-techne.com/images/products/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72846-img0002.jpg "Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846]")
Applications for Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Myeloid leukemia factor 1
Alternate Names
Myelodysplasia-myeloid leukemia factor 1, myeloid leukemia factor 1
Gene Symbol
MLF1
Additional Myeloid leukemia factor 1 Products
Product Documents for Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free
Product Specific Notices for Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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