Myeloperoxidase/MPO Antibody (8F4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51148

Novus Biologicals, part of Bio-Techne
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NBP1-51148
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunoassay, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8F4

Concentration

0.1 mg/ml

View all Myeloperoxidase/MPO Antibodies »

Product Specifications

Immunogen

Purified mouse MPO from WEHI-3 cells

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Positive Control: Neutrophils isolated from digested infarcts Negative control: Lymphocytes isolated from digested infarcts

Scientific Data Images for Myeloperoxidase/MPO Antibody (8F4)

Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148]

Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148]

Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.

Applications for Myeloperoxidase/MPO Antibody (8F4)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500
Application Notes
Use in IHC-P reported in scientific literature (PMID: 26582929).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-51148 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Myeloperoxidase/MPO

The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms. Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.

Alternate Names

MPO

Gene Symbol

MPO

Additional Myeloperoxidase/MPO Products

Product Documents for Myeloperoxidase/MPO Antibody (8F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Myeloperoxidase/MPO Antibody (8F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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