Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41406

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MPO421-8B2

Format

BSA Free

Concentration

1 mg/ml

View all Myeloperoxidase/MPO Antibodies »

Product Specifications

Immunogen

Human myeloperoxidase

Specificity

The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free

Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free [NBP2-41406]

Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free [NBP2-41406]

Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).
Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free [NBP2-41406]

Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free [NBP2-41406]

Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).

Applications for Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-4 ug/ml

Flow Cytometry

1-4 ug/ml
Application Notes
Use in FLOW cytometry reported in scientific literature ( PMID 27355490). This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Myeloperoxidase/MPO

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Alternate Names

MPO

Gene Symbol

MPO

Additional Myeloperoxidase/MPO Products

Product Documents for Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Myeloperoxidase/MPO Antibody (MPO421-8B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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