MYH Antibody (4D10)

Western Blot: MYH Antibody (4D10) [H00004595-M01]

Western Blot: MYH Antibody (4D10) [H00004595-M01] - MUTYH monoclonal antibody (M01), clone 4D10 Analysis of MUTYH expression in Hela S3 NE.

Immunoprecipitation: MYH Antibody (4D10) [H00004595-M01]

Immunoprecipitation: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody.

ELISA: MYH Antibody (4D10) [H00004595-M01]

ELISA: MYH Antibody (4D10) [H00004595-M01] - Detection limit for recombinant GST tagged MUTYH is approximately 1ng/ml as a capture antibody.

Western Blot: MYH Antibody (4D10) [H00004595-M01] -

Western Blot: MYH Antibody (4D10) [H00004595-M01] - Cell death & G1 arrest in hMTH1-depleted cells were rescued by hMYH overexpression. DNA content of cells without (a) or with (b) gamma3-hMYH overexpression at 96 h of Dox treatment is shown in histograms & their corresponding cell cycle distribution are displayed in stacked bar charts in (c, d). The gamma3-hMYH overexpression was validated after 96 h of Dox treatment by western blotting. One representative membrane is displayed in e. The results are presented as mean±s.e.m. from at least two independent experiments in duplicate. P-values were calculated using one-way analysis of variance (ANOVA). *P<0.05, **P<0.01, NS, not significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27918552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MYH Antibody (4D10) [H00004595-M01] -

Western Blot: MYH Antibody (4D10) [H00004595-M01] - Simultaneous suppression of MTH1 & MYH was efficiently achieved using a two-vector system. (a) Steps of cell line establishment are illustrated here. Top 15% of cells expressing GFP & RFP670 were sorted to improve the knockdown efficiency. Expression levels of MYH & MTH1 were analyzed after 96 h of treatment with Dox in shRNA set1 (b) & shRNA set2 (c) using qRT–PCR analysis. Data were normalized to NT-shRNA expressing cells & presented as mean±s.e.m. from three independent experiments in triplicate. To investigate the knockdown efficiency at protein level, western blot analysis was performed after 96 h of Dox treatment of cells with shRNA set1 (d) & shRNA set2 (e). Data were double normalized with GAPDH & NT-shRNA control samples & expressed as percentage. The graphs represent mean±s.e.m. from three independent experiments. P-values were calculated using one-way analysis of variance (ANOVA). *P<0.05, **P<0.01, ***P<0.001, ****P⩽0.0001, NS, not significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27918552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MYH Antibody (4D10) [H00004595-M01] -

Western Blot: MYH Antibody (4D10) [H00004595-M01] - Simultaneous suppression of MTH1 & MYH was efficiently achieved using a two-vector system. (a) Steps of cell line establishment are illustrated here. Top 15% of cells expressing GFP & RFP670 were sorted to improve the knockdown efficiency. Expression levels of MYH & MTH1 were analyzed after 96 h of treatment with Dox in shRNA set1 (b) & shRNA set2 (c) using qRT–PCR analysis. Data were normalized to NT-shRNA expressing cells & presented as mean±s.e.m. from three independent experiments in triplicate. To investigate the knockdown efficiency at protein level, western blot analysis was performed after 96 h of Dox treatment of cells with shRNA set1 (d) & shRNA set2 (e). Data were double normalized with GAPDH & NT-shRNA control samples & expressed as percentage. The graphs represent mean±s.e.m. from three independent experiments. P-values were calculated using one-way analysis of variance (ANOVA). *P<0.05, **P<0.01, ***P<0.001, ****P⩽0.0001, NS, not significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27918552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.