MYH4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05634

Novus Biologicals, part of Bio-Techne
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NBP3-05634-100ul
NBP3-05634-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Myosin Heavy Chain 4/MYH4 Antibodies »

Product Summary for MYH4 Antibody - BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1500-1600 of human MYH4 (NP_060003.2). ETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVES

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYH4 Antibody - BSA Free

Western Blot: MYH4 AntibodyBSA Free [NBP3-05634]

Western Blot: MYH4 AntibodyBSA Free [NBP3-05634]

Western Blot: MYH4 Antibody [NBP3-05634] - Western blot analysis of extracts of C2C12 cells, using MYH4 antibody (NBP3-05634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry: MYH4 Antibody - BSA Free [NBP3-05634]

Immunohistochemistry: MYH4 Antibody - BSA Free [NBP3-05634]

Immunohistochemistry: MYH4 Antibody [NBP3-05634] - Immunofluorescence analysis of mouse skeletal muscle using MYH4 antibody (NBP3-05634) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: MYH4 AntibodyBSA Free [NBP3-05634]

Western Blot: MYH4 AntibodyBSA Free [NBP3-05634]

Western Blot: MYH4 Antibody [NBP3-05634] - Western blot analysis of extracts of Rat skeletal muscle, using MYH4 antibody (NBP3-05634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
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Applications for MYH4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Myosin Heavy Chain 4/MYH4

MYH4, also known as Myosin-4, is a 1939 amino acid protein that is 223 kDa, predominantly found in myocytes mediates plus-ended movement along microfilaments and involved in muscle contraction through cyclic interactions with actin-rich thin filaments, creating a contractile force. It is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations. Current research is being performed on several diseases and disorders including congestive heart failure, yaws, myocarditis, hermaphroditism, liver disease, pharyngitis, and alcoholism. This protein is involved in cell adhesion integrin-mediated cell adhesion and migration, cell adhesion tight junctions, cytoskeleton remodeling regulation of actin cytoskeleton by Rho GTPases, immune response CCR3 signaling in eosinophils, development MAG-dependent inhibition of neurite outgrowth, RhoA, antioxidant action of vitamin-C, transendothelial migration of leukocytes, actin nucleation by ARP-WASP complex, epithelial adherens junctions, inhibitory action of lipoxins on neutrophil migration, membrane trafficking, translocation of GLUT4 to the plasma membrane, translocation of GLUT4 vesicle and docking at the plasma membrane, and Viral myocarditis pathways forming interactions with ESR2, NTHL1, S100A4, USP15, C12orf44, CNBP, ACTB, and over 30 other proteins.

Long Name

Myosin Heavy Chain 4

Alternate Names

MYH2B, MyHC-2b, MyHC-IIb

Gene Symbol

MYH4

Additional Myosin Heavy Chain 4/MYH4 Products

Product Documents for MYH4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MYH4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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