MYL4 Antibody (OTI1H6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46062

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1H6

Concentration

1 mg/ml

View all MYL4 Antibodies »

Product Summary for MYL4 Antibody (OTI1H6)

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-197 of human MYL4(NP_002467) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MYL4 Antibody (OTI1H6)

Western Blot: MYL4 Antibody (OTI1H6) [NBP2-46062]

Western Blot: MYL4 Antibody (OTI1H6) [NBP2-46062]

Western Blot: MYL4 Antibody (1H6) [NBP2-46062] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYL4.
Immunohistochemistry: MYL4 Antibody (OTI1H6) [NBP2-46062]

Immunohistochemistry: MYL4 Antibody (OTI1H6) [NBP2-46062]

Immunohistochemistry: MYL4 Antibody (1H6) [NBP2-46062] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: MYL4 Antibody (OTI1H6) [NBP2-46062]

Western Blot: MYL4 Antibody (OTI1H6) [NBP2-46062]

Western Blot: MYL4 Antibody (1H6) [NBP2-46062] - Analysis of extracts (10ug) from 3 different cell lines and 2 human tissues.
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Applications for MYL4 Antibody (OTI1H6)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MYL4

Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

ALC1, AMLC, GT1, MLC1, Myosin light chain 1, embryonic muscle/atrial isoform, myosin light chain 4, Myosin light chain alkali GT-1 isoform, myosin, atrial/fetal muscle, light chain, myosin, light chain 4, alkali; atrial, embryonic, myosin, light polypeptide 4, alkali; atrial, embryonic, PRO1957

Gene Symbol

MYL4

Additional MYL4 Products

Product Documents for MYL4 Antibody (OTI1H6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MYL4 Antibody (OTI1H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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