MYL4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30954

Novus Biologicals, part of Bio-Techne
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NBP2-30954
NBP2-30954-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MYL4 Antibodies »

Product Summary for MYL4 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PAPEAPKEPAFDPKSVKIDFTADQIE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYL4 Antibody

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining in human heart muscle and skeletal muscle tissues using anti-MYL4 antibody. Corresponding MYL4 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: MYL4 Antibody [NBP2-30954]

Immunocytochemistry/ Immunofluorescence: MYL4 Antibody [NBP2-30954]

Immunocytochemistry/Immunofluorescence: MYL4 Antibody [NBP2-30954] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
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Applications for MYL4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYL4

Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

ALC1, AMLC, GT1, MLC1, Myosin light chain 1, embryonic muscle/atrial isoform, myosin light chain 4, Myosin light chain alkali GT-1 isoform, myosin, atrial/fetal muscle, light chain, myosin, light chain 4, alkali; atrial, embryonic, myosin, light polypeptide 4, alkali; atrial, embryonic, PRO1957

Gene Symbol

MYL4

UniProt

P12829 (Human)

Additional MYL4 Products

Product Documents for MYL4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MYL4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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