MyoD Antibody (5.2F)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-788
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5.2F
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full length Mouse MyoD1 protein.
Epitope
This antibody is specific to an epitope of amino acid 3-56 in mouse MyoD1.
Reactivity Notes
Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Nuclear
Specificity
This antibody is specific to a 45 kD protein, which is indenitified as MyoD1. It does not react with myogenin, Myf5 or Myf6. This antibody stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for MyoD Antibody (5.2F)
![Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]](https://resources.bio-techne.com/images/products/MyoD-Antibody-5-2F-Immunohistochemistry-Paraffin-NB120-788-img0003.jpg "Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]")
Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]
Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788] - Formalin fixed paraffin embedded human rhabdomyosarcoma stained with MyoD antibody (NB120-788).![Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]](https://resources.bio-techne.com/images/products/MyoD-Antibody-5-2F-Immunohistochemistry-Paraffin-NB120-788-img0002.jpg "Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]")
Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]
Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788] - Staining of rhabdomyosarcoma.Applications for MyoD Antibody (5.2F)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Application Notes
IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 30 min at RT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.4), 0.2% BSA, Tween-20
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MyoD
Long Name
Myoblast Determination Protein 1
Alternate Names
bHLHc1, MYF3, MYOD1, PUM
Entrez Gene IDs
17927 (Mouse)
Gene Symbol
MYOD1
Additional MyoD Products
Product Documents for MyoD Antibody (5.2F)
Product Specific Notices for MyoD Antibody (5.2F)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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