MyoD Antibody (JE60-47)

Western Blot: MyoD Antibody (JE60-47) [NBP3-32630]

Western Blot: MyoD Antibody (JE60-47) [NBP3-32630] - Western blot analysis of MyoD on different lysates with Rabbit anti-MyoD antibody (NBP3-32630) at 1/1,000 dilution.

Lane 1: RD cell lysate
Lane 2: HEK-293 cell lysate (negative)
Lane 3: HeLa cell lysate (negative)
Lane 4: RD cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 35 kDa
Observed band size: 45 kDa

Exposure time: 10 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32630) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: MyoD Antibody (JE60-47) [NBP3-32630]

Immunocytochemistry/ Immunofluorescence: MyoD Antibody (JE60-47) [NBP3-32630] - Immunocytochemistry analysis of RD (positive) and HEK-293 (negative) labeling MyoD with Rabbit anti-MyoD antibody (NBP3-32630) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MyoD antibody (NBP3-32630) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.