MyoD Antibody (SPM427) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34772

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM427

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all MyoD Antibodies »

Product Specifications

Immunogen

Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189. (Uniprot: P15172)

Epitope

aa 180-189

Localization

Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Specificity

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this monoclonal antibody maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms sarcomas and alveolar soft part sarcomas.

Marker

Rhabdomyosarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-32882).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MyoD Antibody (SPM427) - Azide and BSA Free

Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) - Azide and BSA Free [NBP2-34772]

Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) - Azide and BSA Free [NBP2-34772]

Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) - Azide and BSA Free [NBP2-34772] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (SPM427).

Applications for MyoD Antibody (SPM427) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MyoD

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Gene Symbol

MYOD1

Additional MyoD Products

Product Documents for MyoD Antibody (SPM427) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MyoD Antibody (SPM427) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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