Myogenin Antibody (F5D) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34616
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Canine, Feline
Applications
CyTOF-ready, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # F5D
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Rat Myogenin peptide (aa 73-94) followed by rat Myogenin recombinant fragment (aa30-224) (Epitope aa138-158) (Uniprot: P15173)
Localization
Nuclear
Specificity
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
Marker
Skeletal Muscle Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32986).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Myogenin Antibody (F5D) - Azide and BSA Free
Immunohistochemistry-Paraffin: Myogenin Antibody (F5D) - Azide and BSA Free [NBP2-34616]
Immunohistochemistry-Paraffin: Myogenin Antibody (F5D) - Azide and BSA Free [NBP2-34616] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with Myogenin MAb (F5D)Flow Cytometry: Myogenin Antibody (F5D) - Azide and BSA Free [NBP2-34616]
Flow Cytometry: Myogenin Antibody (F5D) - Azide and BSA Free [NBP2-34616] - An intracellular stain was performed on RH-30 cells with Myogenin Antibody [F5D] NBP2-34616PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.Applications for Myogenin Antibody (F5D) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5ug/ml for coating
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Myogenin
Alternate Names
BHLHC3, MYF4, MYOG
Gene Symbol
MYOG
Additional Myogenin Products
Product Documents for Myogenin Antibody (F5D) - Azide and BSA Free
Product Specific Notices for Myogenin Antibody (F5D) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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