Myogenin Antibody (JE60-39)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32631

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32631
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE60-39

Concentration

1 mg/ml

View all Myogenin Antibodies »

Product Summary for Myogenin Antibody (JE60-39)

Immunogen

Synthetic peptide within Human Myogenin aa 1-50 / 224. (Uniprot: P15173)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Myogenin Antibody (JE60-39)

Myogenin Antibody (JE60-39)

Western Blot: Myogenin Antibody (JE60-39) [NBP3-32631]

Western Blot: Myogenin Antibody (JE60-39) [NBP3-32631] - Western blot analysis of Myogenin on different lysates with Rabbit anti-Myogenin antibody (NBP3-32631) at 1/1,000 dilution.

Lane 1: Undifferentiated C2C12 whole cell lysate
Lane 2: Differentiated C2C12 for 1 day whole cell lysate
Lane 3: Differentiated C2C12 for 2 days whole cell lysate
Lane 4: Differentiated C2C12 for 3 days whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 25 kDa
Observed band size: 35 kDa

Exposure time: 4 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32631) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Myogenin Antibody (JE60-39)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myogenin

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.

Alternate Names

BHLHC3, MYF4, MYOG

Gene Symbol

MYOG

Additional Myogenin Products

Product Documents for Myogenin Antibody (JE60-39)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Myogenin Antibody (JE60-39)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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