Myoglobin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54897

Novus Biologicals, part of Bio-Techne
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NBP2-54897
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Myoglobin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myoglobin Antibody

Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897]

Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897]

Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897] - Staining of human prostate shows low expression as expected.
Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897]

Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897]

Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897] - Analysis in human skeletal muscle and prostate tissues using Anti-MB antibody. Corresponding MB RNA-seq data are
Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897]

Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897]

Immunohistochemistry-Paraffin: Myoglobin Antibody [NBP2-54897] - Staining of human skeletal muscle shows high expression.

Applications for Myoglobin Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myoglobin

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Alternate Names

MB

Gene Symbol

MB

Additional Myoglobin Products

Product Documents for Myoglobin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Myoglobin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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