Myosin Heavy Chain Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29725

Novus Biologicals, part of Bio-Techne
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NBP3-29725
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Myosin Heavy Chain Antibodies »

Product Specifications

Immunogen

Between 1850 and 1900

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin Heavy Chain Antibody

Myosin Heavy Chain Antibody

Immunoprecipitation:Myosin Heavy Chain AntibodyNBP3-29725] -

Immunoprecipitation:Myosin Heavy Chain AntibodyNBP3-29725] -Detection of human MYH14 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MYH14 antibody NBP3-29725 (lot NBP3-29725-1) used for IP at 6 µg per reaction. MYH14 was also immunoprecipitated by rabbit anti-MYH14.. For blotting immunoprecipitated MYH14, NBP3-29725 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Myosin Heavy Chain Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Myosin Heavy Chain

Myosin heavy chain is a muscle myosin, which is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac myosin heavy chain exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the myosin heavy chain genes are associated with several different dilated and hypertrophic cardiomyopathies.

Alternate Names

MYHC

Gene Symbol

MYH14

Additional Myosin Heavy Chain Products

Product Documents for Myosin Heavy Chain Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myosin Heavy Chain Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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