Myosin Light Chain 2 Antibody (3B2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30249
![Western Blot: Myosin Light Chain 2 Antibody (3B2)BSA Free [NBP1-30249]](https://resources.bio-techne.com/images/products/Myosin-Light-Chain-2-Antibody-3B2-Western-Blot-NBP1-30249-img0001.jpg "Western Blot: Myosin Light Chain 2 Antibody (3B2)BSA Free [NBP1-30249]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Xenopus
Cited:
Human, Mouse, Frog - Xenopus (African Clawed Frog)
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3B2
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human Myosin Light Chain 2 (1-166aa) purified from E. coli (NP_000423).
Reactivity Notes
Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Myosin Light Chain 2 Antibody (3B2) - BSA Free
Western Blot: Myosin Light Chain 2 Antibody (3B2)BSA Free [NBP1-30249]
Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse heart tissue lysate![Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]](https://resources.bio-techne.com/images/products/Myosin-Light-Chain-2-Antibody-3B2-Immunocytochemistry-Immunofluorescence-NBP1-30249-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]")
Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]
Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).![Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]](https://resources.bio-techne.com/images/products/Myosin-Light-Chain-2-Antibody-3B2-Flow-Cytometry-NBP1-30249-img0003.jpg "Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]")
Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]
Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Applications for Myosin Light Chain 2 Antibody (3B2) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1x10^6cells
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:1000-1:2000
Application Notes
Use in IHC and ICC/IF was reported in a scientific publication (PMID: 23727888).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using NBP1-30249 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Myosin Light Chain 2
Alternate Names
cardiac ventricular myosin light chain 2, CMH10myosin light chain 2, DKFZp779C0562, MLC2, MLC-2, MLC-2v, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, RLC of myosin, slow cardiac myosin regulatory light chain 2
Gene Symbol
MYL2
UniProt
Additional Myosin Light Chain 2 Products
Product Documents for Myosin Light Chain 2 Antibody (3B2) - BSA Free
Product Specific Notices for Myosin Light Chain 2 Antibody (3B2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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