Myosin Light Chain 2 Antibody (3B2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30249

Novus Biologicals, part of Bio-Techne
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NBP1-30249
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Xenopus

Cited:

Human, Mouse, Frog - Xenopus (African Clawed Frog)

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3B2

Format

BSA Free

Concentration

1.0 mg/ml

View all Myosin Light Chain 2 Antibodies »

Product Specifications

Immunogen

Recombinant human Myosin Light Chain 2 (1-166aa) purified from E. coli (NP_000423).

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Myosin Light Chain 2 Antibody (3B2) - BSA Free

Western Blot: Myosin Light Chain 2 Antibody (3B2)BSA Free [NBP1-30249]

Western Blot: Myosin Light Chain 2 Antibody (3B2)BSA Free [NBP1-30249]

Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse heart tissue lysate
Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]

Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]

Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]

Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) - BSA Free [NBP1-30249]

Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for Myosin Light Chain 2 Antibody (3B2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1x10^6cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:1000-1:2000
Application Notes
Use in IHC and ICC/IF was reported in a scientific publication (PMID: 23727888).

Reviewed Applications

Read 1 review rated 3 using NBP1-30249 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin Light Chain 2

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.

Alternate Names

cardiac ventricular myosin light chain 2, CMH10myosin light chain 2, DKFZp779C0562, MLC2, MLC-2, MLC-2v, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, RLC of myosin, slow cardiac myosin regulatory light chain 2

Entrez Gene IDs

4633 (Human); 363925 (Rat)

Gene Symbol

MYL2

UniProt

P10916 (Human)

Additional Myosin Light Chain 2 Products

Product Documents for Myosin Light Chain 2 Antibody (3B2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Myosin Light Chain 2 Antibody (3B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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