Myosin Light Chain 2 Antibody (3B9-B4)
Novus Biologicals, part of Bio-Techne | Catalog # H00004633-M01
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3B9-B4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MYL2 (AAH15821.1, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD
Specificity
MYL2 - myosin, light polypeptide 2, regulatory, cardiac, slow
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Myosin Light Chain 2 Antibody (3B9-B4)
Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01]
Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of monoclonal antibody to MYL2 on HeLa cell . Antibody concentration 10 ug/ml.Immunoprecipitation: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01]
Immunoprecipitation: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of MYL2 transfected lysate using anti-MYL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYL2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Detection limit for recombinant GST tagged MYL2 is 0.03 ng/ml as a capture antibody.
Applications for Myosin Light Chain 2 Antibody (3B9-B4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:10-1:500
Sandwich ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Myosin Light Chain 2
Alternate Names
cardiac ventricular myosin light chain 2, CMH10myosin light chain 2, DKFZp779C0562, MLC2, MLC-2, MLC-2v, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, RLC of myosin, slow cardiac myosin regulatory light chain 2
Entrez Gene IDs
4633 (Human)
Gene Symbol
MYL2
OMIM
160781 (Human)
UniProt
Additional Myosin Light Chain 2 Products
Product Documents for Myosin Light Chain 2 Antibody (3B9-B4)
Product Specific Notices for Myosin Light Chain 2 Antibody (3B9-B4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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