Myosin Light Chain 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99552

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Myosin Light Chain 2 Antibodies »

Product Summary for Myosin Light Chain 2 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Myosin Light Chain 2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Myosin Light Chain 2 Antibody

Western Blot: Myosin Light Chain 2 Antibody [NBP2-99552]

Western Blot: Myosin Light Chain 2 Antibody [NBP2-99552]

Western Blot: Myosin Light Chain 2 Antibody [NBP2-99552] - Anti-Myosin Light Chain 2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse heart tissue lysate Lane B: Mouse muscle tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa
Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552] - Immunochemical staining of mouse Myosin Light Chain 2 in rat skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552]

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody [NBP2-99552] - Immunochemical staining of mouse Myosin Light Chain 2 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
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Applications for Myosin Light Chain 2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin Light Chain 2

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Alternate Names

cardiac ventricular myosin light chain 2, CMH10myosin light chain 2, DKFZp779C0562, MLC2, MLC-2, MLC-2v, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, RLC of myosin, slow cardiac myosin regulatory light chain 2

Gene Symbol

MYL2

Additional Myosin Light Chain 2 Products

Product Documents for Myosin Light Chain 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myosin Light Chain 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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