Myosin light chain 3 Antibody (4C2)
Novus Biologicals, part of Bio-Techne | Catalog # H00004634-M12
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4C2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MYL3 (AAH09790, 1 a.a. ~ 195 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPKKPEPKKDDAKAAPKAAPAPAPPPEPERPKEVEFDASKIKIEFTPEQIEEFKEAFMLFDRTPKCEMKITYGQCGDVLRALGQNPTQAEVLRVLGKPRQEELNTKMMDFETFLPMLQHISKNKDTGTYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGERLTEDEVEKLMAGQEDSNGCINYEAFVKHIMSS
Specificity
MYL3 - myosin, light polypeptide 3, alkali; ventricular, skeletal, slow (4C2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Myosin light chain 3 Antibody (4C2)
![Western Blot: Myosin light chain 3 Antibody (4C2) [H00004634-M12]](https://resources.bio-techne.com/images/products/Myosin-light-chain-3-Antibody-4C2-Western-Blot-H00004634-M12-img0003.jpg "Western Blot: Myosin light chain 3 Antibody (4C2) [H00004634-M12]")
Western Blot: Myosin light chain 3 Antibody (4C2) [H00004634-M12]
Western Blot: Myosin light chain 3 Antibody (4C2) [H00004634-M12] - Analysis of MYL3 expression in transfected 293T cell line by MYL3 monoclonal antibody (M12), clone 4C2. Lane 1: MYL3 transfected lysatE (21.9 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Myosin light chain 3 Antibody (4C2) [H00004634-M12] - Detection limit for recombinant GST tagged MYL3 is approximately 3ng/ml as a capture antibody.
Applications for Myosin light chain 3 Antibody (4C2)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Myosin light chain 3
Alternate Names
Cardiac myosin light chain 1, CMH8Ventricular/slow twitch myosin alkali light chain, CMLC1, light polypeptide 3, alkali; ventricular, skeletal, slow, MLC1SBMyosin light chain 1, slow-twitch muscle B/ventricular isoform, MLC1V, myosin light chain 3, myosin, light chain 3, alkali; ventricular, skeletal, slow, VLC1
Entrez Gene IDs
4634 (Human)
Gene Symbol
MYL3
UniProt
Additional Myosin light chain 3 Products
Product Documents for Myosin light chain 3 Antibody (4C2)
Product Specific Notices for Myosin light chain 3 Antibody (4C2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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