Myosin Phosphatase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75097
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for Myosin Phosphatase Antibody
Immunogen
Peptide with sequence C-REDEYKQKYSRTYD, from the C Terminus of the protein sequence according to NP_002471.1, NP_001231919.1, NP_001231921 .1, NP_001137358.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Myosin Phosphatase Antibody
Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] -
Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP2-75097] - (4ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] -
Immunocytochemistry/Immunofluorescence: Myosin Phosphatase Antibody [NBP2-75097] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: Myosin Phosphatase Antibody [NBP2-75097] -
Flow Cytometry: Myosin Phosphatase Antibody [NBP2-75097] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for Myosin Phosphatase Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Immunohistochemistry-Paraffin
4 - 5 ug/ml
Peptide ELISA
1:16000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
TBS (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Myosin Phosphatase
Alternate Names
MBSmyosin phosphatase, target subunit 1, MGC133042, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, MYPT1protein phosphatase 1 regulatory subunit 12A, protein phosphatase 1, regulatory (inhibitor) subunit 12A, Protein phosphatase myosin-binding subunit
Gene Symbol
PPP1R12A
Additional Myosin Phosphatase Products
Product Documents for Myosin Phosphatase Antibody
Product Specific Notices for Myosin Phosphatase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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