Myosin Phosphatase [p Thr853] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38643

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Myosin Phosphatase Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around T853 of human Myosin Phosphatase (NP_002471.1).

Sequence:
RSTGV

Modification

p Thr 853

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Myosin Phosphatase [p Thr853] Antibody

Myosin Phosphatase [p Thr853] Antibody

Western Blot: Myosin Phosphatase [p Thr853] Antibody [NBP3-38643] -

Western Blot: Myosin Phosphatase [p Thr853] Antibody [NBP3-38643] - Western blot analysis of various lysates using Myosin Phosphatase Rabbit pAb ( AP0916) at 1:1000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37C for 1 hour. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for Myosin Phosphatase [p Thr853] Antibody

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Myosin Phosphatase

The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component act as a regulatory subunit known as myosine binding (MBS) or myosine phosphatae targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoform of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.

Alternate Names

MBSmyosin phosphatase, target subunit 1, MGC133042, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, MYPT1protein phosphatase 1 regulatory subunit 12A, protein phosphatase 1, regulatory (inhibitor) subunit 12A, Protein phosphatase myosin-binding subunit

Gene Symbol

PPP1R12A

Additional Myosin Phosphatase Products

Product Documents for Myosin Phosphatase [p Thr853] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Myosin Phosphatase [p Thr853] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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