Myosin VB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87746

Novus Biologicals, part of Bio-Techne
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NBP1-87746
NBP1-87746-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Simple Western, Western Blot

Cited:

Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Myosin VB Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLK

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26201991). Use in Rat reported in scientific literature (PMID:31757887).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin VB Antibody

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues . Corresponding MYO5B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium, placenta, skeletal muscle and small intestine using Anti-MYO5B antibody NBP1-87746 (A) shows similar protein distribution across tissues to independent antibody NBP1-87747 (B).
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
View 4 more images

Applications for Myosin VB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 27229121)

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Knockout Validated

Validation (PMID: 31664880)

Simple Western

1:100

Western Blot

Reported in scientific literature (PMID: 27229121)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin VB

Myosin and Kinesin motor domain. These ATPases belong to the P-loop NTPase family and provide the driving force in myosin and kinesin mediated processes.

Alternate Names

KIAA1119, MYO5B variant protein, myosin VB, myosin-Vb

Gene Symbol

MYO5B

Additional Myosin VB Products

Product Documents for Myosin VB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Myosin VB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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