MYST1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55251
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human males absent on the first (MYST histone acetyltransferase 1) using the numbering given in entry BAB13924.1 (GeneID 84148).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MYST1 Antibody
Western Blot: MYST1 Antibody [NB100-55251]
Western Blot: MYST1 Antibody [NB100-55251] - Detection of human MOF/MYST1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody NB100-55251 (lot NB100-55251-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). MOF/MYST1 was also immunoprecipitated by a previous lot (lot NB100-55251-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).Immunohistochemistry-Paraffin: MYST1 Antibody [NB100-55251]
Immunohistochemistry-Paraffin: MYST1 Antibody [NB100-55251] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiMOF/MYST1 used at a dilution of 1:1,000 (1ug/ml). Detection: DABWestern Blot: MYST1 Antibody [NB100-55251]
Western Blot: MYST1 Antibody [NB100-55251] - Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for MYST1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2 -5 ug/mg lysate
Western Blot
1:1000 -1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MYST1
Alternate Names
FLJ14040, hMOFhistone acetyltransferase MYST1, KAT8, MOFEC 2.3.1.48, MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1, MYST histone acetyltransferase 1, MYST-1, ortholog of Drosophila males absent on the first (MOF), probable histone acetyltransferase MYST1
Entrez Gene IDs
84148 (Human)
Gene Symbol
KAT8
UniProt
Additional MYST1 Products
Product Documents for MYST1 Antibody
Product Specific Notices for MYST1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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