N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10)

Novus Biologicals, part of Bio-Techne | Catalog # H00003340-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibodies »

Product Specifications

Immunogen

NDST1 (NP_001534, 38 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LYGWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALI

Specificity

NDST1 - N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10)

Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01]

Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01]

Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - NDST1 monoclonal antibody (M01), clone 1G10 Analysis of NDST1 expression in A-549.
Immunohistochemistry-Paraffin: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01]

Immunohistochemistry-Paraffin: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01]

Immunohistochemistry-Paraffin: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - Analysis of monoclonal antibody to NDST1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01]

Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01]

Western Blot: N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10) [H00003340-M01] - Western Blot analysis of NDST1 expression in A-549 ( Cat # L025V1 ).
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Applications for N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: N-Deacetylase/N-Sulfotransferase 1/NDST1

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine(GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backboneto make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a rolein determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence isabsolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands,thereby playing a role in inflammatory response

Alternate Names

HSNST1, HSST1, NST1

Entrez Gene IDs

3340 (Human)

Gene Symbol

NDST1

OMIM

600853 (Human)

UniProt

NP_001534 (Human)

Additional N-Deacetylase/N-Sulfotransferase 1/NDST1 Products

Product Documents for N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for N-Deacetylase/N-Sulfotransferase 1/NDST1 Antibody (1G10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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