n-Myc Antibody (NMYC-1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB200-109

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), CyTOF-ready, Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Flow Cytometry, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # NMYC-1

Format

BSA Free

Concentration

1.0 mg/ml

View all n-Myc Antibodies »

Product Specifications

Immunogen

A synthetic peptide (SPYVESEDAPPQKC) conjugated to KLH, corresponding to amino acids 327-339 of human n-Myc. [UniProt# P04198]

Localization

Nuclear, some cytoplasmic.

Specificity

This antibody does not react with c-Myc or L-Myc.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for n-Myc Antibody (NMYC-1) - BSA Free

Western Blot: n-Myc Antibody (NMYC-1)BSA Free [NB200-109]

Western Blot: n-Myc Antibody (NMYC-1)BSA Free [NB200-109]

Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: n-Myc Antibody (NMYC-1) - BSA Free [NB200-109]

Immunocytochemistry/ Immunofluorescence: n-Myc Antibody (NMYC-1) - BSA Free [NB200-109]

Immunocytochemistry/Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Western Blot: n-Myc Antibody (NMYC-1)BSA Free [NB200-109]

Western Blot: n-Myc Antibody (NMYC-1)BSA Free [NB200-109]

Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in human heart tissue.

Applications for n-Myc Antibody (NMYC-1) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10 - 1:500. Use reported in scientific literature (PMID 21068393)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Flow Cytometry

reported in scientific literature (PMID 27539729)

Gel Super Shift Assays

1:1 - 1:100. Use reported in scientific literature (PMID 21068393)

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunoprecipitation

1:10 - 1:100

Western Blot

1:500 - 1:1000
Application Notes
In Western blot a band can been seen at ~50-65 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: n-Myc

n-Myc, also known as bHLHe37, NMYC, N-myc proto-oncogene protein, and MYCN, is a transcriptional regulator of cell proliferation, differentiation and apoptosis. n-Myc is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Defects in n-Myc are the cause of Feingold syndrome type 1 (FGLDS1), a syndrome characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability, mental retardation, and limb malformations. n-Myc expression is highest during embryonic development and then later in the adult during B-cell development. These expression patterns and results from targeted deletion of n-Myc suggest that n-Myc plays an important role in tissue development and differentiation. Recent research has linked n-Myc to suppressing the anti-apoptotic protein Bcl-2 (PMID: 22920753), as well as elucidated this proteins role in various forms of tumorigenesis (PMID: 22722710).

Alternate Names

BHLHE37, bHLHe37N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, MODED, neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene, N-myc, NMYCneuroblastoma MYC oncogene, ODED, oncogene NMYC, pp65/67, v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived, v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Entrez Gene IDs

4613 (Human); 18109 (Mouse)

Gene Symbol

MYCN

UniProt

P04198 (Human)

Additional n-Myc Products

Product Documents for n-Myc Antibody (NMYC-1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for n-Myc Antibody (NMYC-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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