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N myc interactor Antibody (9D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00009111-M01

Novus Biologicals, part of Bio-Techne
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H00009111-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 9D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NMI (AAH01268, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI

Reactivity Notes

Human. Other species not tested.

Specificity

NMI - N-myc (and STAT) interactor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for N myc interactor Antibody (9D8)

Western Blot: N myc interactor Antibody (9D8) [H00009111-M01]

Western Blot: N myc interactor Antibody (9D8) [H00009111-M01]

Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - NMI monoclonal antibody (M01), clone 9D8 Analysis of NMI expression in HeLa.
Immunohistochemistry-Paraffin: N myc interactor Antibody (9D8) [H00009111-M01]

Immunohistochemistry-Paraffin: N myc interactor Antibody (9D8) [H00009111-M01]

Immunohistochemistry-Paraffin: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: N myc interactor Antibody (9D8) [H00009111-M01]

Western Blot: N myc interactor Antibody (9D8) [H00009111-M01]

Western Blot: N myc interactor Antibody (9D8) [H00009111-M01] - Analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi ( Cat # H00009111-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody (M01), clone 9D8 (Cat # H00009111-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for N myc interactor Antibody (9D8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: N myc interactor

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Alternate Names

Nmi, N-myc (and STAT) interactor, N-myc and STAT interactor, N-myc interactor, N-myc-interactor

Entrez Gene IDs

9111 (Human)

Gene Symbol

NMI

OMIM

603525 (Human)

Additional N myc interactor Products

Product Documents for N myc interactor Antibody (9D8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for N myc interactor Antibody (9D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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