N-WASP Antibody (4P5M9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16103

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16103-100ul
NBP3-16103-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4P5M9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all N-WASP Antibodies »

Product Summary for N-WASP Antibody (4P5M9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human N-WASP (O00401). LPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N-WASP Antibody (4P5M9)

Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103]

Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103]

Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103] - Western blot analysis of extracts of HeLa cells, using N-WASP/N-WASP Rabbit mAb (NBP3-16103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: N-WASP Antibody (4P5M9) [NBP3-16103]

Immunohistochemistry-Paraffin: N-WASP Antibody (4P5M9) [NBP3-16103]

Immunohistochemistry-Paraffin: N-WASP Antibody (4P5M9) [NBP3-16103] - Immunohistochemistry of paraffin-embedded mouse kidney using N-WASP/N-WASP Rabbit mAb (NBP3-16103) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103]

Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103]

Western Blot: N-WASP Antibody (4P5M9) [NBP3-16103] - Western blot analysis of extracts of various cell lines, using N-WASP/N-WASP Rabbit mAb (NBP3-16103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Applications for N-WASP Antibody (4P5M9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: N-WASP

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Gene Symbol

WASL

Additional N-WASP Products

Product Documents for N-WASP Antibody (4P5M9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for N-WASP Antibody (4P5M9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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