N-WASP Antibody

Western Blot: N-WASP Antibody [NBP1-82512]

Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in human cell line RT-4.

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512]

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human cerebral cortex shows moderate to strong positivity in neuronal cells.

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512]

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human kidney shows moderate to strong positivity in cells in tubules and glomeruli.

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512]

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human skeletal muscle shows weak positivity in myocytes.

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512]

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human stomach shows moderate to strong positivity in glandular cells.

Simple Western: N-WASP Antibody [NBP1-82512]

Simple Western: N-WASP Antibody [NBP1-82512] - Simple Western lane view shows a specific band for N-WASP in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Knockdown Validated: N-WASP Antibody [NBP1-82512]

Knockdown Validated: N-WASP Antibody [NBP1-82512] - Generation of N-WASP knockdown cell lines. A-549 and SK-MES-1 cells were treated with N-WASP siRNA/non-targeting siRNA (NT) at 0.5 ug/ml and then analysed for N-WASP expression after 48 h. A QPCR analysis of N-WASP transcript expression demonstrates a significant decrease in expression in siRNA treated cells, n = 4 replicates. b) PCR also demonstrates a decrease in N-WASP expression. d) Representative image showing knockdown of N-WASP at protein level in siRNA treated cells at 48 h. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3219-3) licensed under a CC-BY license.

Western Blot: N-WASP Antibody [NBP1-82512] -

Western Blot: N-WASP Antibody [NBP1-82512] - Generation of nWASP knockdown cell lines. A-549 & SK-MES-1 cells were treated with nWASP siRNA/non-targeting siRNA (NT) at 0.5 μg/ml & then analysed for nWASP expression after 48 h. a QPCR analysis of nWASP transcript expression demonstrates a significant decrease in expression in siRNA treated cells, n = 4 replicates. b PCR also demonstrates a decrease in nWASP expression. c Quantitative analysis of Western blots (n = 4) shows significant decrease in nWASP protein expression in both A-549 & SK-MES1 cell lines after 48 h siRNA treatment. d Representative image showing knockdown of nWASP at protein level in siRNA treated cells at 48 h Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28351346), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: N-WASP Antibody [NBP1-82512] -

Western Blot: N-WASP Antibody [NBP1-82512] - nWASP activity affects A-549 & SK-MES-1 cell growth. a, b nWASP inhibition using 10 μM wiskostatin treatment significantly impairs the growth of A-549 & SK-MES-1 cells, respectively. c, d A significant effect on growth is also evident after 3 days in nWASP knockdown A-549 & SK-MES-1 cells, respectively, when compared to non-targeting control treated cells Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28351346), licensed under a CC-BY license. Not internally tested by Novus Biologicals.