Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all N4BP1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1. Amino Acid Squence: TAPAEKAELLEQSRGRIE

Specificity

At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N4BP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531]

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531]

Immunocytochemistry/Immunofluorescence: N4BP1 Antibody [NBP1-76531] - Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 ug/mL.
N4BP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531] -

Immunocytochemistry/ Immunofluorescence: N4BP1 Antibody - BSA Free [NBP1-76531] - Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 ug/mL.
N4BP1 Antibody - BSA Free

Western Blot: N4BP1 Antibody - BSA Free [NBP1-76531] -

Western Blot: N4BP1 Antibody - BSA Free [NBP1-76531] - WB Validation in Human Cell LinesLoading: 15 ug of lysates Antibodies: N4BP1, 1 ug/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 sec

Applications for N4BP1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: N4BP1

Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

Alternate Names

FLJ31821, KIAA0615, MGC176730, NEDD4 binding protein 1, NEDD4-binding protein 1

Gene Symbol

N4BP1

UniProt

NP_694574 (Human)

Additional N4BP1 Products

Product Documents for N4BP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for N4BP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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