Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all N4BP1 Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 250 to 300 of human NEDD4 Binding Protein 1 using the numbering given in entry NP_694574.3 (GeneID 9683).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N4BP1 Antibody

Western Blot: N4BP1 Antibody [NBP2-37688]

Western Blot: N4BP1 Antibody [NBP2-37688]

Western Blot: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: N4BP1 Antibody [NBP2-37688]

Immunoprecipitation: N4BP1 Antibody [NBP2-37688]

Immunoprecipitation: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for IP at 6 ug per reaction. N4BP1 was also immunoprecipitated by rabbit anti-N4BP1 antibody BL17672. For blotting immunoprecipitated N4BP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for N4BP1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: N4BP1

Alternate Names

FLJ31821, KIAA0615, MGC176730, NEDD4 binding protein 1, NEDD4-binding protein 1

Gene Symbol

N4BP1

UniProt

NP_694574.3 (Human)

Additional N4BP1 Products

Product Documents for N4BP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for N4BP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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