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NACA2 Antibody (4H6)

Novus Biologicals, part of Bio-Techne | Catalog # H00342538-M01

Novus Biologicals, part of Bio-Techne
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H00342538-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NACAL (NP_954984, 116 a.a. ~ 215 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ASDAYIVFGEAKIQDLSQQAQLAAAEKFRVQGEAVGNIQENTQTPTVQEESEEEEVDETGVEVKDVKLVMSQANVSRAKAVRALKNNSNDIVNAIMELTV

Specificity

NACAL - nascent-polypeptide-associated complex alpha polypeptide-like (4H6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NACA2 Antibody (4H6)

Western Blot: NACA2 Antibody (4H6) [H00342538-M01]

Western Blot: NACA2 Antibody (4H6) [H00342538-M01]

Western Blot: NACA2 Antibody (4H6) [H00342538-M01] - Analysis of NACA2 expression in transfected 293T cell line by NACAL monoclonal antibody (M01), clone 4H6.Lane 1: NACA2 transfected lysate (Predicted MW: 23.2 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: NACA2 Antibody (4H6) [H00342538-M01] - Detection limit for recombinant GST tagged NACA2 is 3 ng/ml as a capture antibody.

Applications for NACA2 Antibody (4H6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NACA2

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds tonascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognitionparticle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity ofribosomes for protein translocation sites in the ER membrane (M sites)

Alternate Names

alpha-NAC protein, Alpha-NAC-like, ANAC, Hom s 2.01, hom s 2.01 protein, IgE autoantigen alpha-nascent polypeptide-associated complex, MGC71999, NACAL, NAC-alpha-like, nascent polypeptide-associated complex alpha subunit 2, nascent polypeptide-associated complex subunit alpha-2, Nascent polypeptide-associated complex subunit alpha-like, nascent polypeptide-associated complex, alpha polypeptide, 2, nascent-polypeptide-associated complex alpha polypeptide-like

Entrez Gene IDs

342538 (Human)

Gene Symbol

NACA2

Additional NACA2 Products

Product Documents for NACA2 Antibody (4H6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NACA2 Antibody (4H6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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