NAIP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77196

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, E. coli

Cited:

Bacteria - Escherichia coli

Predicted:

Mouse (92%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunomicroscopy, Immunoprecipitation, SDS-Page, Western Blot

Cited:

Immunomicroscopy, Immunoprecipitation, SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all NAIP Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP. The immunogen is located within amino acids 1200 - 1250 of NAIP. Amino Acid Squence: QFPDEETSEKFAY

Reactivity Notes

E. coli reactivity reported in scientific literature (PMID: 31551961).

Specificity

Minor, lower molecular weight bands may represent alternately spliced forms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NAIP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196]

Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196]

Immunocytochemistry/Immunofluorescence: NAIP Antibody [NBP1-77196] - Confocal microscopy analysis of NAIP expression in THP-1m with or without CFT073 stimulation. NLRC4 and NAIP expression in THP-1m, 6 h post stimulation with or without (mock) CFT073 was examined with confocal microscopy analysis using anti-NAIP antibody. Alexa fluor 488 (green) was used to detect anti-NAIP antibody. Nucleus of cells were stained with DAPI (blue). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.
Western Blot: NAIP AntibodyBSA Free [NBP1-77196]

Western Blot: NAIP AntibodyBSA Free [NBP1-77196]

Western Blot: NAIP Antibody [NBP1-77196] - Analysis of NLRP3, NLRC4, NAIP, AIM2, ASC, Caspase-4, Caspase-1, B-actin and GAPDH protein expression by immunoblotting. (A) Represents the immunoblotting results of NLRP3, NLRC4, NAIP, Caspase-1, GAPDH of UPEC infected UTI patients and controls group. Scattered plots showing individual densitometric values (IDV) of NAIP (D). Results were expressed as average densitometric ratio in patients and controls group +/- SD. P-value is ***p = 0.0001 and n.s, non-significant. anti-NLRP3 antibody (NBP1-77080), anti-NLRC4 antibody (NBP2-41124), anti-Caspase-1 antibody (NBP1-45433). Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.
Western Blot: NAIP AntibodyBSA Free [NBP1-77196]

Western Blot: NAIP AntibodyBSA Free [NBP1-77196]

Western Blot: NAIP Antibody [NBP1-77196] - Analysis of NAIP in human tonsil tissue lysate with NAIP antibody at 1 ug/mL.
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Applications for NAIP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Western Blot

0.5-1 ug/ml
Application Notes
Use in SDS- Page , Immunoprecipitation and Immunomicroscopy reported in scientific literature (PMID:31551961).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NAIP

Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules (reviewed in 3). Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.

Long Name

Neuronal Apoptosis Inhibitor Protein

Alternate Names

BIRC1, NLRB1

Gene Symbol

NAIP

UniProt

AAC52047 (Human)

Additional NAIP Products

Product Documents for NAIP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NAIP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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