NALP12 Antibody - BSA Free

Western Blot: NALP12 AntibodyBSA Free [NBP1-76293]

Western Blot: NALP12 Antibody [NBP1-76293] - B6 mice (n = 6) were infected ocularly with 2.5 x 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 and KOS63 (mock-infected mice (n = 6) used as controls). 48 hrs later, animals were euthanized & corneas were excised & homogenized in lysis buffer, and WB was performed for NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 (beta-actin used as a control). Three WB performed using different corneal lysates harvested from three sets of corneas from 2 mice (4 corneas/lysate). Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, and AIM2 at 48 hrs post-infection compared to controls, and normalized to beta-actin. Representative densitometric analysis is shown compared to control. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/articles/10.3389/fimmu.2019.01631/full), licensed under a CC-BY license.

Western Blot: NALP12 AntibodyBSA Free [NBP1-76293]

Western Blot: NALP12 Antibody [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 and AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent and less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with clinical isolates KOS79 and KOS63 at MOI of 1. Two, 8 and 24 h post-infection, hTCEpi cells were harvested and immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, and AIM2 inflammasomes. Corresponding b-actin was used as a control. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2019.01631/full), licensed under a CC-BY license.

Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] -

Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 & AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent & less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with HSV-1 laboratory strains McKrae, 17, F, RE or KOS (A,B) or with clinical isolates KOS79 & KOS63 (C,D) at MOI of 1. Two, 8 & 24 h post-infection, hTCEpi cells were harvested & immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, & AIM2 inflammasomes. Corresponding beta-actin was used as a control. (B,D) Graphs show the kinetics of fold changes in NLRP3, NLRP6, NLRP12, IFI16, & AIM2 inflammasomes expression at 2, 8, & 24 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control & normalized to beta-actin. Results are representative of three experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31367214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] -

Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Expression levels of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 inflammasomes in mouse corneas following infection with virulent & less-virulent strains of HSV-1. B6 mice (n = 6) were infected ocularly with 2.5 × 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 & KOS63. Mock-infected mice (n = 6) were used as controls. Forty-eight hours post-infection animals were euthanized the corneas were excised & homogenized in lysis buffer, & western blot was performed for expression of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2. Corresponding beta-actin was used as a control. Three western blot were performed using three different corneal lysates harvested from three sets of corneas pooled from 2 infected mice (i.e., 4 corneas per lysate). Results are representative of those three independent experiments. Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 at 48 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control & normalized to beta-actin. Representative densitometric analysis is shown compared to control. *P < 0.05; **P < 0.01; ***P < 0.001 using Student's t test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31367214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.