Nanog Antibody (CL5803) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44196
![Nanog Antibody (CL5803) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP3-44196]](https://resources.bio-techne.com/images/products/nbp3-44196_mouse-nanog-mab-cl5803-azide-and-bsa-free-28220251324585.jpg "Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP3-44196]")
![Nanog Antibody (CL5803) - Azide and BSA Free Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]](https://resources.bio-techne.com/images/products/nbp3-44196_mouse-nanog-mab-cl5803-azide-and-bsa-free-282202512182712.jpg "Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL5803
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of Q9H9S0, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Nanog Antibody (CL5803) - Azide and BSA Free
Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]
Staining of human bladder cancer shows absence of positivity in tumor cells as expected (negative control).![Nanog Antibody (CL5803) - Azide and BSA Free Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]](https://resources.bio-techne.com/images/products/nbp3-44196_mouse-nanog-mab-cl5803-azide-and-bsa-free-282202513261973.jpg "Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]")
Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]
Staining of human testis cancer shows strong nuclear immunoreactivity in tumor cells.![Nanog Antibody (CL5803) - Azide and BSA Free Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]](https://resources.bio-techne.com/images/products/nbp3-44196_mouse-nanog-mab-cl5803-azide-and-bsa-free-282202513332377.jpg "Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]")
Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44196]
Staining of human testis shows absence of positivity as expected (negative control).Applications for Nanog Antibody (CL5803) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nanog
Long Name
Nanog Homeobox
Alternate Names
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Gene Symbol
NANOG
Additional Nanog Products
Product Documents for Nanog Antibody (CL5803) - Azide and BSA Free
Product Specific Notices for Nanog Antibody (CL5803) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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