Nanog Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13177

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot (Negative)

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Nanog Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region of the mouse Nanog protein (within residues 1-50). [Swiss-Prot Q80Z64 ]

Localization

Nucleus.

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nanog Antibody - BSA Free

Immunohistochemistry-Paraffin: Nanog Antibody - BSA Free [NBP2-13177]

Immunohistochemistry-Paraffin: Nanog Antibody - BSA Free [NBP2-13177]

Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - Staining of Nanog in mouse testis using DAB with hematoxylin counterstain.

Applications for Nanog Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanog

In stem cell development, the influence of the transcription factor Nanog is important for appropriate differentiation, or conversely to maintain their pluripotent state. Nanog is important for stem cell growth, maintenance and differentiation, but is usually co-expressed and co-activated by other factors. Islet survivor cells, generated for progenitor cell testing, have nuclear expression of OCT4, SOX2 and Nanog, indicating a probability that they can maintain pluripotent characteristics in experimentation. In brain cancer, there are some stem cell like behaviors, and the cancer cells exhibiting these characteristics demonstrate high concentrations of Nanog, influenced by p53.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Entrez Gene IDs

71950 (Mouse)

Gene Symbol

NANOG

UniProt

Q80Z64

Additional Nanog Products

Product Documents for Nanog Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nanog Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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