Nanos2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76917

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Nanos2 Antibodies »

Product Summary for Nanos2 Antibody - BSA Free

Immunogen

Antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of the human Nanos2. The immunogen is located within the first 50 amino acids of Nanos2. Amino Acid Squence: SRGQRLETGEIEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nanos2 Antibody - BSA Free

Western Blot: Nanos2 AntibodyBSA Free [NBP1-76917]

Western Blot: Nanos2 AntibodyBSA Free [NBP1-76917]

Western Blot: Nanos2 Antibody [NBP1-76917] - Rat brain tissue lysate with Nanos2 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Nanos2 Antibody - BSA Free [NBP1-76917]

Immunocytochemistry/ Immunofluorescence: Nanos2 Antibody - BSA Free [NBP1-76917]

Immunocytochemistry/Immunofluorescence: Nanos2 Antibody [NBP1-76917] - Immunofluorescence of Nanos2 in Human Testis cells with Nanos2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Nanos2 Antibody - BSA Free [NBP1-76917]

Immunohistochemistry-Paraffin: Nanos2 Antibody - BSA Free [NBP1-76917]

Immunohistochemistry-Paraffin: Nanos2 Antibody [NBP1-76917] - Human brain tissue cells with Nanos2 antibody at 2.5 ug/ml.

Applications for Nanos2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanos2

Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos2 antibody will not cross-react with either Nanos 1 or Nanos3.

Alternate Names

nanos homolog 2, nanos homolog 2 (Drosophila), NOS2NOS-2

Gene Symbol

NANOS2

UniProt

P60321

Additional Nanos2 Products

Product Documents for Nanos2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nanos2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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