NANS Antibody (3G6)

Novus Biologicals, part of Bio-Techne | Catalog # H00054187-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NANS Antibodies »

Product Specifications

Immunogen

NANS (NP_061819, 260 a.a. ~ 359 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AELVRSVRLVERALGSPTKQLLPCEMACNEKLGKSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPEDIFNLVGKKVLVTVEEDDTIMEELVDNHGKKIKS

Reactivity Notes

Human. Other species not tested.

Specificity

NANS - N-acetylneuraminic acid synthase (sialic acid synthase)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NANS Antibody (3G6)

Western Blot: NANS Antibody (3G6) [H00054187-M01]

Western Blot: NANS Antibody (3G6) [H00054187-M01]

Western Blot: NANS Antibody (3G6) [H00054187-M01] - NANS monoclonal antibody (M01), clone 3G6 Analysis of NANS expression in HeLa.
Sandwich ELISA: NANS Antibody (3G6) [H00054187-M01] - Detection limit for recombinant GST tagged NANS is approximately 0.03ng/ml as a capture antibody.

Applications for NANS Antibody (3G6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NANS

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Alternate Names

EC 2.5.1.56, EC 2.5.1.57, N-acetylneuraminate synthase, N-acetylneuraminate-9-phosphate synthase, N-acetylneuraminic acid phosphate synthase, N-acetylneuraminic acid synthasesialic acid phosphate synthase, SASsialic acid synthase

Entrez Gene IDs

54187 (Human)

Gene Symbol

NANS

OMIM

605202 (Human)

UniProt

NP_061819 (Human)

Additional NANS Products

Product Documents for NANS Antibody (3G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NANS Antibody (3G6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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