NAPA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32493

Novus Biologicals, part of Bio-Techne
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NBP1-32493
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Porcine (97%), Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NAPA Antibodies »

Product Summary for NAPA Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NAPA. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (89%),Chimpanzee (100%).

Localization

Membrane, Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NAPA Antibody

Western Blot: NAPA Antibody [NBP1-32493]

Western Blot: NAPA Antibody [NBP1-32493]

Western Blot: NAPA Antibody [NBP1-32493] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with alpha SNAP antibody [N2C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: NAPA Antibody [NBP1-32493]

Immunocytochemistry/ Immunofluorescence: NAPA Antibody [NBP1-32493]

Immunocytochemistry/Immunofluorescence: NAPA Antibody [NBP1-32493] - DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha SNAP protein stained by alpha SNAP antibody [N2C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: NAPA Antibody [NBP1-32493]

Immunohistochemistry-Paraffin: NAPA Antibody [NBP1-32493]

Immunohistochemistry-Paraffin: NAPA Antibody [NBP1-32493] - Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] ) dilution: 1:500.
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Applications for NAPA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500-1:10000
Application Notes
Immunoprecipitation Assay-dependent dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NAPA

NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues. Recombinant human NAPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

alpha-SNAP, alpha-soluble NSF attachment protein, N-ethylmaleimide-sensitive factor attachment protein alpha, N-ethylmaleimide-sensitive factor attachment protein, alpha, S, SNAPA, SNAP-alpha

Gene Symbol

NAPA

Additional NAPA Products

Product Documents for NAPA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NAPA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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