Napsin A Antibody (NAPSA/1238)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45244

Novus Biologicals, part of Bio-Techne
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NBP2-54383
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NAPSA/1238

Concentration

0.2 mg/ml

View all Napsin A Antibodies »

Product Specifications

Immunogen

Recombinant human Napsin A protein fragment (around aa189-299) (exact sequence is proprietary) (Uniprot: O96009)

Localization

Cytoplasmic

Marker

Lung Adenocarcinoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-54383)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Napsin A Antibody (NAPSA/1238)

Western Blot: Napsin A Antibody (NAPSA/1238) [NBP2-45244]

Western Blot: Napsin A Antibody (NAPSA/1238) [NBP2-45244]

Western Blot: Napsin A Antibody (NAPSA/1238) [NBP2-45244] - Western Blot of K562, HEK293 and A549 cell Lysates using Napsin-A Monoclonal Antibody (NAPSA/1238)
Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1238) [NBP2-45244]

Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1238) [NBP2-45244]

Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1238) [NBP2-45244] - Human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238).
Protein Array: Napsin A Antibody (NAPSA/1238) [NBP2-45244]

Protein Array: Napsin A Antibody (NAPSA/1238) [NBP2-45244]

Protein Array: Napsin A Antibody (NAPSA/1238) [NBP2-45244] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Napsin A Antibody (NAPSA/1238). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Napsin A Antibody (NAPSA/1238)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Napsin A

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Alternate Names

ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02

Gene Symbol

NAPSA

UniProt

O96009

Additional Napsin A Products

Product Documents for Napsin A Antibody (NAPSA/1238)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Napsin A Antibody (NAPSA/1238)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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