Napsin A Antibody (TMU-Ad02) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80866

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TMU-Ad02

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Napsin A Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the N-terminus of human NAPSA [UniProt# O96009]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Napsin A Antibody (TMU-Ad02) - Azide and BSA Free

Western Blot: Napsin A Antibody (TMU-Ad02)Azide and BSA Free [NBP2-80866]

Western Blot: Napsin A Antibody (TMU-Ad02)Azide and BSA Free [NBP2-80866]

Western Blot: Napsin A Antibody (TMU-Ad02) - Azide and BSA Free [NBP2-80866] - Analysis of NAPSA expression in human lung using NB110-68133. Image from the standard format of this antibody.
Immunohistochemistry: Napsin A Antibody (TMU-Ad02) - Azide and BSA Free [NBP2-80866]

Immunohistochemistry: Napsin A Antibody (TMU-Ad02) - Azide and BSA Free [NBP2-80866]

Immunohistochemistry: Napsin A Antibody (TMU-Ad02) - Azide and BSA Free [NBP2-80866] - Analysis of NAPSA on human lung adenocarcinoma using NB110-68133. Image from the standard format of this antibody.

Applications for Napsin A Antibody (TMU-Ad02) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

0.5-1 ug/ml

Immunohistochemistry-Paraffin

0.5-1 ug/ml

Western Blot

0.5-1 ug/ml
Application Notes
This NAPSA antibody is useful for Western blot and Immunohistochemistry on formalin-fixed, paraffin embedded sections. In WB a band can be seen around 45-50 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Napsin A

NAPSA (Napsin A) is an aspartic proteinase that belongs to peptidase A1 family (NAPSA, cathepsins D and E, renin, pepsin, gastricsin, and bovine chymosin) and it is primarily expressed in lung and kidney, and at a very low levels in spleen as well as in peripheral blood leukocytes. NAPSA's expression is regulated by TTF-1 which also regulates the expression of surfactant protein B. Inside the lung, NAPSA is expressed in alveolar type II pneumocytes where it colocalize with prosurfactant B protein as well as surfactant B in the lamellar bodies, and has been suggested to be involved in N-terminal/C-terminal processing of prosurfactant B protein. NAPSA is also localized in intra-alveolar macrophages, probably as a result of phagocytosis. In renal tissues, NAPSA is expressed in the proximal tubules, where it is suggested to function as a lysosomal protease involved in protein catabolism.

Alternate Names

ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02

Gene Symbol

NAPSA

Additional Napsin A Products

Product Documents for Napsin A Antibody (TMU-Ad02) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Napsin A Antibody (TMU-Ad02) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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