NARFL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83611

Novus Biologicals, part of Bio-Techne
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NBP1-83611
NBP1-83611-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NARFL Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLEASRPDFFNQEHQTRDVDCVLTTGEVFRLLEEEGVSLPDLEPAPLDSLCSGASAEEPTSHRGGGSGGYLEHVFRHAARELFGIHVAEVTYKP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NARFL Antibody

Western Blot: NARFL Antibody [NBP1-83611]

Western Blot: NARFL Antibody [NBP1-83611]

Western Blot: NARFL Antibody [NBP1-83611] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: NARFL Antibody [NBP1-83611]

Immunocytochemistry/ Immunofluorescence: NARFL Antibody [NBP1-83611]

Immunocytochemistry/Immunofluorescence: NARFL Antibody [NBP1-83611] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: NARFL Antibody [NBP1-83611]

Immunohistochemistry-Paraffin: NARFL Antibody [NBP1-83611]

Immunohistochemistry-Paraffin: NARFL Antibody [NBP1-83611] - Staining of human kidney shows strong cytoplasmic positivity in distal tubules.

Applications for NARFL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NARFL

NARFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation ofextramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effectcould be indirect

Alternate Names

cytosolic Fe-S cluster assembly factor NARFL, FLJ21988, HPRN, IOP1Protein related to Narf, Iron-only hydrogenase-like protein 1, LET1 like/JFP15, nuclear prelamin A recognition factor-like, Nuclear prelamin A recognition factor-like protein, PRNLET1L

Gene Symbol

CIAO3

Additional NARFL Products

Product Documents for NARFL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NARFL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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