NARS2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82077
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LAEFYMIEAEISFVDSLQDLMQVIEELFKATTMMVLSKCPEDVELCHKFIAPGQKDRLEHMLKNNFLIISYTEAVEILKQASQNFTFTPEWGADLRTEHEKYLVKHCGNIPVFVINYPLTLKPFYMRDNEDGPQHTVAAVDLLVPGVG
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NARS2 Antibody
Western Blot: NARS2 Antibody [NBP1-82077]
Western Blot: NARS2 Antibody [NBP1-82077] - Analysis in human cell line K562.Immunocytochemistry/ Immunofluorescence: NARS2 Antibody [NBP1-82077]
Immunocytochemistry/Immunofluorescence: NARS2 Antibody [NBP1-82077] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & mitochondria. Antibody staining is shown in green.Immunohistochemistry-Paraffin: NARS2 Antibody [NBP1-82077]
Immunohistochemistry-Paraffin: NARS2 Antibody [NBP1-82077] - Staining of human kidney shows strong cytoplasmic positivity with a granular pattern in tubule cells.Applications for NARS2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Simple Western
1:20
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 review rated 4 using NBP1-82077 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NARS2
Alternate Names
AsnRS, asparagine tRNA ligase 2, mitochondrial (putative), Asparagine--tRNA ligase, asparaginyl-tRNA synthetase 2, mitochondrial (putative), EC 6.1.1, EC 6.1.1.22, FLJ23441, probable asparaginyl-tRNA synthetase, mitochondrial, SLM5
Gene Symbol
NARS2
Additional NARS2 Products
Product Documents for NARS2 Antibody
Product Specific Notices for NARS2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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