Native Lipoteichoic Acid Antibody (55)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-60146

Novus Biologicals, part of Bio-Techne
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NBP1-60146-0.025mg
NBP1-60146-0.1mg
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Key Product Details

Species Reactivity

Validated:

Bacteria

Cited:

Bacteria

Applications

Flow Cytometry, Functional, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 55

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Native Lipoteichoic Acid Antibodies »

Product Specifications

Immunogen

Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977

Reactivity Notes

The specificity is labled not applicable. Bacteria reactivity reported in scientific literature (PMID:33055259).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Description

Please See references for verifying information below:
IA: For detection, 1.2 ug/ml antibody in PBS was added for 1 hr at 37 C on LTA coated plates
FC: 1/250
IF: 60' in PBS/0.02%BSA/0.02% saponin
W: A reduced or native sample treatment and run on 15% SDS-Page. Blot was incubated o/n at 4C with a 1/1000 dilution .The band size is ~17 kDa

Applications for Native Lipoteichoic Acid Antibody (55)

Application
Recommended Usage

Flow Cytometry

1:250

Immunoassay

1.2 ug/ml in PBS

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Culture medium + 0.02% NaN3

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Native Lipoteichoic Acid

The monoclonal antibody 55 recognizes lipoteichoic acid (LTA). LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2. Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.

Alternate Names

LTA

Additional Native Lipoteichoic Acid Products

Product Documents for Native Lipoteichoic Acid Antibody (55)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Native Lipoteichoic Acid Antibody (55)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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