Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # N68/6

Concentration

1 mg/ml

View all Nav1.7 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1751-1946 (C-terminus) of human Nav1.7

Localization

Membrane , Synapse

Specificity

Detects approx 230kDa. No cross-reactivity against other Nav channels.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nav1.7 Antibody (N68/6)

Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904]

Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904]

Western Blot: Nav1.7 Antibody (N68/6) [NBP2-12904] - Western Blot analysis of Hamster CHO cells showing detection of Nav1.7 Sodium Channel protein using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904]

Immunocytochemistry/ Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904]

Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.7 Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav1.7 Monoclonal Antibody (NBP2-12904) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.7 Antibody (D) Composite.
Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]

Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904]

Immunohistochemistry: Nav1.7 Antibody (N68/6) [NBP2-12904] - Immunohistochemistry analysis using Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody, Clone N68/6 (NBP2-12904). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.7 Sodium Channel Monoclonal Antibody (NBP2-12904) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
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Applications for Nav1.7 Antibody (N68/6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of Nav1.7 Antibody was sufficient for detection of Nav1.7 in 10 ug of HEK-293 cell lysate transiently expressing Nav1.7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nav1.7

1 ug/mL was sufficient for detection of Nav1.7 in 10ug of HEK-293 cell lysate transiently expressing Nav1.7 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

Sodium channel protein type 9 subunit alpha

Alternate Names

hNE-Na, Ne-Na, NENA, PN1, SCN9A

Gene Symbol

SCN9A

Additional Nav1.7 Products

Product Documents for Nav1.7 Antibody (N68/6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nav1.7 Antibody (N68/6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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